Facilities

 

Peng Lab is designed to be a unique research environment that is a blend of computational biology and genomics with experimental research in infectious disease. The College provided us the start-up fund for setting up a high-performance computational infrastructure, which is fully integrated into NC State Bioinformatics Research Center (BRC) computing cluster system and is supported by dedicated BRC IT staff. In our College’s Research Building, we have independent bench space in the regular open laboratory, plus equipment space in shared laboratories inside the building and the full access to a shared BSL-3 lab on the same floor. We have set up our laboratory equipment like Bioanalyzer, PCR machines, gel electrophoresis system and imaging system, and plate reader, in addition to the access to College’s shared equipment such as Illumina’s MiniSeq sequencer and Applied Biosystems’s Real-Time PCR System. We also have an Illumina NextSeq 500 sequencer, a high-throughput benchtop next-generation sequencing unit installed in our Lab. The Lab has the set up for preparing BSL-3 level samples for deep sequencing analysis. All generated deep sequencing data is directly transferred to our dedicated file server at BRC and backed up online plus remote long-term data archive. More recently, we have set up a 10X Chromium system for single cell analysis, in collaboration with several groups at NC State (Ghashghaei, Cheng, Piedrahita, Aylor).

BSL-3 Laboratory A shared BSL-3 lab gives us the capacity to safely prepare infectious samples for deep sequencing analysis.	10X Chromium The Chromium System enables multiple high-throughput single cell analysis such as single cell RNA-seq and immune repertoire profiling.	Illumina Nextseq 500 The NextSeq 500 has the capacity to produce up to 800 million paired-end reads for high throughput DNA and RNA sequencing.	Computer Cluster All deep sequencing data is directly transferred to our dedicated file server at the BRC and backed up online and remotely.